A new molecular marker system,
presence/absence variation (PAV) of large fragment genomic sequences shows
potential because of its convenient PCR-based technique and distinct band
distribution on. However, its application in soybean breeding and genetic
research remains to be developed.
Source: Baidu
There are some core parents in soybean breeding program, which are the key to
success in breeding, revealing their role is helpful for parent selection in
hybrid breeding work. In a study, 154 soybean materials including 28 parents
and 126 breeding lines were used to reveal their genetic relationship.
These
lines were derived from the crosses between three core parents (Nannong86.4,
Nannong88.48 and Youchu4) and a set of domestic and exotic lines in a breeding
program in National Center of Soybean Improvement.
The average number of allele per PAV was 2.1. The average value of polymorphic
information content (PIC) per PAV was 0.239. The average allele number of the
gene-based and non-coding region PAV marker and the average values of
polymorphic information content (PIC) of the two PAV types were almost the
same.
All 34 crosses were clustered into 6 groups based on PAV markers data. Among
them, three big groups correspond to the three core parents could be
identified. All 154 materials were clustered into 9 groups. Most lines from the
same core parent or same cross were classified as a group. According to the
genetic contribution rate of PAV allele of the core parents, 22 crosses had
higher average contribution rate than the expected ratio in the 29 crosses.
The
observed contribution values of core parent Nannong86.4, Nannong88.48 were
higher than the expected values based on pedigree analysis, while Youchu4 from
other region had less contribution to the derived lines, indicating the
rationalities of using local elite line as core parent in breeding program. In
comparison with pedigree analysis, PAV marker can reveal detail genetic
relationship among the parents and their derived lines.
This article comes from Seed China News 1608, CCM
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Tag: soybean